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fbs-free dmem  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fbs-free dmem
    Fbs Free Dmem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fbs-free+dmem/fbs+free+dmem/pmc11913433-113-8-10
    Average 90 stars, based on 1 article reviews
    fbs-free dmem - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: G6PD-mediated increase in de novo NADP + biosynthesis promotes antioxidant defense and tumor metastasis.
    Article Snippet: Briefly, cells with or without siRNA treatment were cultured in FBS-free DMEM (Thermo Fisher Scientific) for 4 hours.

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 h, the growth medium was replaced with fresh, FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition.
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham’s F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification-mediated upregulation and modulation of the PEBP1-RAF1 interaction
    Article Snippet: ESCC cells were seeded (1×10 5 ) in FBS-free DMEM (Thermo Fisher Scientific, Inc.) at 37°C in the upper chamber of Matrigel-coated inserts (BD Biosciences).

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 hours, the growth medium was replaced with fresh FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: LncRNA-COX2 inhibits Fibroblast Activation and Epidural Fibrosis by Targeting EGR1
    Article Snippet: The plasmid of LncRNA-COX2, shRNA- LncRNA-COX2 or shRNA-EGR1 were separately mixed with Lipotectamine 2000 (Invitrogen, Carlsbad, CA, USA) and FBS-Free DMEM (Invitrogen, Carlsbad, CA, USA) for 20 min. And then the mixtures were added to different groups of fibroblasts for 8 hours.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham's F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: ADAM17-mediated EGFR ligand shedding directs macrophage-promoted cancer cell invasion.
    Article Snippet: Upon rehydration of the precoated Matrigel invasion chambers (pore size 8 μm, Corning), 0.5 × 105 to 2 × 105 DiI-labeled cancer cells were seeded in 500 μL FBS-free DMEM (Gibco, Thermo Fisher Scientific) in the upper chamber of the inserts with or without 10% polarized or treated BMDMs or 50% polarized or IL-4–differentiated THP-1 macrophages.

    Derivative Assay:

    Article Title: G6PD-mediated increase in de novo NADP + biosynthesis promotes antioxidant defense and tumor metastasis.
    Article Snippet: Briefly, cells with or without siRNA treatment were cultured in FBS-free DMEM (Thermo Fisher Scientific) for 4 hours.

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 h, the growth medium was replaced with fresh, FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition.
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham’s F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification-mediated upregulation and modulation of the PEBP1-RAF1 interaction
    Article Snippet: ESCC cells were seeded (1×10 5 ) in FBS-free DMEM (Thermo Fisher Scientific, Inc.) at 37°C in the upper chamber of Matrigel-coated inserts (BD Biosciences).

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 hours, the growth medium was replaced with fresh FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: LncRNA-COX2 inhibits Fibroblast Activation and Epidural Fibrosis by Targeting EGR1
    Article Snippet: The plasmid of LncRNA-COX2, shRNA- LncRNA-COX2 or shRNA-EGR1 were separately mixed with Lipotectamine 2000 (Invitrogen, Carlsbad, CA, USA) and FBS-Free DMEM (Invitrogen, Carlsbad, CA, USA) for 20 min. And then the mixtures were added to different groups of fibroblasts for 8 hours.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham's F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: ADAM17-mediated EGFR ligand shedding directs macrophage-promoted cancer cell invasion.
    Article Snippet: Upon rehydration of the precoated Matrigel invasion chambers (pore size 8 μm, Corning), 0.5 × 105 to 2 × 105 DiI-labeled cancer cells were seeded in 500 μL FBS-free DMEM (Gibco, Thermo Fisher Scientific) in the upper chamber of the inserts with or without 10% polarized or treated BMDMs or 50% polarized or IL-4–differentiated THP-1 macrophages.

    Ex Vivo:

    Article Title: G6PD-mediated increase in de novo NADP + biosynthesis promotes antioxidant defense and tumor metastasis.
    Article Snippet: Briefly, cells with or without siRNA treatment were cultured in FBS-free DMEM (Thermo Fisher Scientific) for 4 hours.

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 h, the growth medium was replaced with fresh, FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition.
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham’s F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification-mediated upregulation and modulation of the PEBP1-RAF1 interaction
    Article Snippet: ESCC cells were seeded (1×10 5 ) in FBS-free DMEM (Thermo Fisher Scientific, Inc.) at 37°C in the upper chamber of Matrigel-coated inserts (BD Biosciences).

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 hours, the growth medium was replaced with fresh FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: LncRNA-COX2 inhibits Fibroblast Activation and Epidural Fibrosis by Targeting EGR1
    Article Snippet: The plasmid of LncRNA-COX2, shRNA- LncRNA-COX2 or shRNA-EGR1 were separately mixed with Lipotectamine 2000 (Invitrogen, Carlsbad, CA, USA) and FBS-Free DMEM (Invitrogen, Carlsbad, CA, USA) for 20 min. And then the mixtures were added to different groups of fibroblasts for 8 hours.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham's F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: ADAM17-mediated EGFR ligand shedding directs macrophage-promoted cancer cell invasion.
    Article Snippet: Upon rehydration of the precoated Matrigel invasion chambers (pore size 8 μm, Corning), 0.5 × 105 to 2 × 105 DiI-labeled cancer cells were seeded in 500 μL FBS-free DMEM (Gibco, Thermo Fisher Scientific) in the upper chamber of the inserts with or without 10% polarized or treated BMDMs or 50% polarized or IL-4–differentiated THP-1 macrophages.

    Plasmid Preparation:

    Article Title: G6PD-mediated increase in de novo NADP + biosynthesis promotes antioxidant defense and tumor metastasis.
    Article Snippet: Briefly, cells with or without siRNA treatment were cultured in FBS-free DMEM (Thermo Fisher Scientific) for 4 hours.

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 h, the growth medium was replaced with fresh, FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition.
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham’s F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification-mediated upregulation and modulation of the PEBP1-RAF1 interaction
    Article Snippet: ESCC cells were seeded (1×10 5 ) in FBS-free DMEM (Thermo Fisher Scientific, Inc.) at 37°C in the upper chamber of Matrigel-coated inserts (BD Biosciences).

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 hours, the growth medium was replaced with fresh FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: LncRNA-COX2 inhibits Fibroblast Activation and Epidural Fibrosis by Targeting EGR1
    Article Snippet: The plasmid of LncRNA-COX2, shRNA- LncRNA-COX2 or shRNA-EGR1 were separately mixed with Lipotectamine 2000 (Invitrogen, Carlsbad, CA, USA) and FBS-Free DMEM (Invitrogen, Carlsbad, CA, USA) for 20 min. And then the mixtures were added to different groups of fibroblasts for 8 hours.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham's F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: ADAM17-mediated EGFR ligand shedding directs macrophage-promoted cancer cell invasion.
    Article Snippet: Upon rehydration of the precoated Matrigel invasion chambers (pore size 8 μm, Corning), 0.5 × 105 to 2 × 105 DiI-labeled cancer cells were seeded in 500 μL FBS-free DMEM (Gibco, Thermo Fisher Scientific) in the upper chamber of the inserts with or without 10% polarized or treated BMDMs or 50% polarized or IL-4–differentiated THP-1 macrophages.

    shRNA:

    Article Title: G6PD-mediated increase in de novo NADP + biosynthesis promotes antioxidant defense and tumor metastasis.
    Article Snippet: Briefly, cells with or without siRNA treatment were cultured in FBS-free DMEM (Thermo Fisher Scientific) for 4 hours.

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 h, the growth medium was replaced with fresh, FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition.
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham’s F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: MTHFD2 promotes esophageal squamous cell carcinoma progression via m6A modification-mediated upregulation and modulation of the PEBP1-RAF1 interaction
    Article Snippet: ESCC cells were seeded (1×10 5 ) in FBS-free DMEM (Thermo Fisher Scientific, Inc.) at 37°C in the upper chamber of Matrigel-coated inserts (BD Biosciences).

    Article Title: Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity
    Article Snippet: After 24 hours, the growth medium was replaced with fresh FBS-free DMEM (Invitrogen) supplemented with 0.1% penicillin and streptomycin.

    Article Title: LncRNA-COX2 inhibits Fibroblast Activation and Epidural Fibrosis by Targeting EGR1
    Article Snippet: The plasmid of LncRNA-COX2, shRNA- LncRNA-COX2 or shRNA-EGR1 were separately mixed with Lipotectamine 2000 (Invitrogen, Carlsbad, CA, USA) and FBS-Free DMEM (Invitrogen, Carlsbad, CA, USA) for 20 min. And then the mixtures were added to different groups of fibroblasts for 8 hours.

    Article Title: Hypothalamic JNK1-hepatic fatty acid synthase axis mediates a metabolic rewiring that prevents hepatic steatosis in male mice treated with olanzapine via intraperitoneal: Additional effects of PTP1B inhibition
    Article Snippet: Cells were plated in 6- or 12-well collagen IV (C3867-1VL, Sigma-Aldrich) pre-coated plates and cultured in medium containing DMEM and Ham's F-12 medium (1:1) with heat-inactivated 10% fetal bovine serum (FBS, 10270–106, Life Technologies, Carlsbad, CA, USA) supplemented with 2 mM glutamine (25030–024, Life Technologies), 15 mM glucose, 20 mM HEPES, 100 U/ml penicillin, 100 μg/ml streptomycin (15140–122, Life Technologies) and 1 mM sodium pyruvate (11360–039, Life Technologies) (attachment media) for 24 h. Primary mouse hepatocytes derived from non-treated mice were exposed ex vivo to different concentrations of OLA for 48 h in FBS-free DMEM (11966–025, Life Technologies) supplemented with 5.5 mM glucose.

    Article Title: ADAM17-mediated EGFR ligand shedding directs macrophage-promoted cancer cell invasion.
    Article Snippet: Upon rehydration of the precoated Matrigel invasion chambers (pore size 8 μm, Corning), 0.5 × 105 to 2 × 105 DiI-labeled cancer cells were seeded in 500 μL FBS-free DMEM (Gibco, Thermo Fisher Scientific) in the upper chamber of the inserts with or without 10% polarized or treated BMDMs or 50% polarized or IL-4–differentiated THP-1 macrophages.



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